Human Sorcin (SRI) ELISA Kit from MyBioSource.com

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Human Sorcin (SRI) ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Skin Reactive Factor, Inflammatory Factor (also known as SRF/IF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SRF/IF antibody. SRF/IF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SRF/IF Antibody is added and binds to SRF/IF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SRF/IF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SRF/IF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm